The UFC confirmed the matchup on Tuesday night.
It had been discussed for days, but required Henderson's medical clearance, a hurdle which has apparently been bypassed.
Both fighters will be looking to rebound into the win column.
At UFC 157, "Hendo" returned after more than a year away to a knee injury but lost a split-decision to Lyoto Machida. The defeat not only snapped his four-fight win streak, but also took him out of the running for the division's top contender spot. Meanwhile, Evans (17-3-1) is looking to snap a two-fight losing skid, with decision defeats to current 205-pound champion Jon Jones, and more recently, Antonio Rogerio Nogueira.
The two light-heavyweights are among two other matches that have been officially announced by the promotion. One is another light-heavyweight fight that will pit Mauricio "Shogun" Rua against Antonio Rogerio Nogueira, and the other is a heavyweight matchup of Stipe Miocic against newly signed Soa Palelei.
UFC 161 will mark the promotion's first-ever trip to Winnipeg, Manitoba, Canada. The event will take place on June 15 at the MTS Centre.